![]() ![]() Besides, different concentrations of both berberine chloride and barberry ethanolic extract showed to have no growth inhibitory effect on normal blood cells (PBMC). Inhibitory effect of berberis crude extract on a-glucosidase was more potent than that of berberine chloride, while both had the same AChE inhibitory effect. ![]() Barberry extract showed potent antioxidative capacity through decreasing TBARS, NO and the oxidation of DPPH that associated with GPx and SOD hyperactivation. Results: Our results showed that barberry crude extract contains 0.6 mg berberine/mg crude extract. On the other hand, the effect of extract and berberine as anticancer was estimated on three different cell lines which were MCF-7, HepG-2, and Caco-2 cells by using neutral red uptake assay which compared with control normal cells (PBMC). vulgaris extract and berberine chloride on cellular thiobarbituric acid reactive species (TBARS) formation, diphenyle-a-picrylhydrazyl (DPPH) oxidation, cellular nitric oxide (NO) radical scavenging capability, superoxide dismutase (SOD), glutathione peroxidase (GPx), acetylcholinesterase (AChE) and a-gulcosidase activities were spectrophotometrically determined. The aims of this study was to bioscreen and compare the in vitro biological activity (antioxidant, cholinergic, antidaibetic and the anticancer) of barberry crude extract and berberine active compound. In vitro biological assessment of berberis vulgaris and its active constituent, berberine: antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effectsĪbeer E Abd El-Wahab2, Doaa A Ghareeb1,2*, Eman EM Sarhan3, Marwa M Abu-Serie2 and Maha A El Demellawy2īackground: Berberis vulgaris is a well known plant with traditional herbal medical history. ![]()
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